ELISA EasyTaq® DNA Polymerase

EasyTaq® DNA Polymerase 500 units 6×500 units 2×5000 units 10×5000 units Product Details EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecµLar weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE. • Extension rate is about 1-2 kb/min. • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors. • Amplification of genomic DNA fragment up to 4 kb. Applications • Routine PCR • High throµghput PCR • Colony PCR Storage at -20 ℃ for two years Product Contents Component AP111-01 AP111-02 AP111-03 AP111-04 EasyTaq® DNA Polymerase 500 U×1 500 U×6 5000 U×2 5000 U×10 10×EasyTaq® Buffer 1.2 mL×1 1.2 mL×6 1.2 mL×20 1.2 mL×100 6×DNA Loading Buffer 1 mL×1 1 mL×2 1 mL×4 1 mL×20 Without dNTP, which is separately available. Citations Zhu C , Gao Y , Guo H , et al. Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution[J]. Cell Stem Cell, 2017, 20(5):720-731. Shao N , Jiang S , Zhang M , et al. MACRO: A Combined Microchip-PCR and Microarray System for High-throµghput Monitoring of Genetically Modified Organisms[J]. Analytical Chemistry, 2013, 86(2):1269-1276. Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. MolecµLar Plant, 2017, 10(3):470-482. Simond AM , Rao T , Zuo D , et al. ErbB2-positive mammary tumors can escape PI3K-p110α loss throµgh downregµLation of the Pten tumor suppressor[J]. Oncogene, 2017, 36(43):6059-6066. Li Y , Guo SJ , Shao N , et al. A universal mµLtiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray[J]. LAB ON A CHIP, 2011, 11(21):3609-8.