ELISA Phospho-SPHK1 (Ser225) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:SPHK1 GeneID:8877 Uniprot:Q9NYA1 Specificity:Phospho-SPHK1 (Ser225) Antibody detects endogenous levels of SPHK1 only when phosphorylated at Ser225. Immunogen:A syntheQuantityd peptide derived from SPHK1 around the phosphorylation site of Ser225. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Prediction: Application:WB,ELISA(peptide) Dilution:WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2840380 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF8318-BP Des# Blocking Peptide:Phospho-SPHK1 (Ser225) Blocking peptide Blocking Peptide Price:$549 CAD