ELISA Phospho-p27 Kip1 (Thr187) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:CDKN1B GeneID:1027 Uniprot:P46527 Specificity:Phospho-p27 Kip1 (Thr187) Antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Threonine 187. Immunogen:A syntheQuantityd peptide derived from p27 Kip1 around the phosphorylation site of Thr187. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction:Bovine,Sheep,Rabbit,Dog,Chicken Application:WB,IHC,IF/ICC,ELISA(peptide) Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2834743 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF3324-BP Des# Blocking Peptide:Phospho-p27 Kip1 (Thr187) Blocking peptide Blocking Peptide Price:$549 CAD