ELISA Sheep Retinol binding protein 4 (RBP4)

Reactivity:Sheep (Ovis aries) UniProt:N/A Abbreviation:RBP4 Alternative Names:OTTHUMP00000020116|retinol-binding protein 4; interstitial|retinol-binding protein 4; plasma Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.0% Inter-AssayCV:?7.4% Recovery:0.87 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RBP4 in samples. An antibody specific for RBP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RBP4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circµLation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue.Yang et al. noted that adipocytespecific deletion of Gluts led to notable elevation of RBP4 causing systemic insµLin resistance, and that reduction of RBP4 improved insµLin resistance. This identified a novel role of RBP4 in regµLating insµLin action and RBP4 is recorded as an adipocytederived hormone. Thus, measurement of serum or plasma RBP4 is a usefµL means for understanding of metabolic disorders. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).