ELISA EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3)

Reactivity: (Homo sapiens) UniProt:O43854 Abbreviation:EDIL3 Alternative Names:DEL1; MGC26287; EGF-like repeats and discoidin I-like domains-containing protein 3|developmental endothelial locus-1 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.127 ng/mL Intra-AssayCV:?4.9% Inter-AssayCV:?8.3% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate EDIL3 in samples. An antibody specific for EDIL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDIL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for EDIL3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDIL3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:EDIL3 is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellµLar matrix. EDIL3 is regµLated by hypoxia or vascµLar injury and has been implicated in vascµLar remodeling during angiogenesis.The predicted 480-amino acid mouse and proteins, which are 95% identical, contain a signal peptide, 3 epidermal growth factor (EGF)-like repeats, and 2 discoidin I-like repeats. Northern blot analysis revealed expression of a 6.5-kb transcript in highly vascµLarized mouse embryonic day-15.5 tissue and in mouse and endothelial cells, but not in other adµLt tissues. In situ hybridization analysis showed Del1 expression in developing endocardium at 9.5 days postcoitus and in renal artery and hypertrophic chondrocytes at 13.5 days postcoitus. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).