ELISA LIM domain transcription factor LMO4 (LMO4)

Reactivity: (Homo sapiens) UniProt:P61968 Abbreviation:LMO4 Alternative Names:OTTHUMP00000011906 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.115 ng/mL Intra-AssayCV:?6.7% Inter-AssayCV:?10.3% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LMO4 in samples. An antibody specific for LMO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LMO4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMO4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:LMO4 was expressed predominantly in the lobµLoalveoli of the mammary gland during pregnancy. Consistent with a role in proliferation, forced expression of this gene inhibited differentiation of mammary epithelial cells. Overexpression of LMO4 mRNA was observed in 5 of 10 breast cancer cell lines. Moreover, in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LMO4 in 56% of specimens. Immunohistochemistry confirmed overexpression in a high percentage (62%) of tumors. These studies implied a role for LMO4 in maintaining proliferation of mammary epithelium and sµggested that deregµLation of this gene may contribute to breast tumorigenesis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).