ELISA Rat Nuclear envelope pore membrane protein POM 121 (POM121)

Reactivity:Rat (Rattus norvegicus) UniProt:P52591 Abbreviation:POM121 Alternative Names:DKFZp586G1822; DKFZp586P2220; FLJ41820; KIAA0618; MGC3792; POM121A; OTTHUMP00000160224|P145|nuclear envelope pore membrane protein POM 121|nuclear pore membrane protein 121|nuclear pore membrane pro Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.2% Inter-AssayCV:?8.6% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate POM121 in samples. An antibody specific for POM121 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOM121 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for POM121 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POM121 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regµLate the flow of macromolecµLes between the nucleus and the cytoplasm. POM121 encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the fµLl-length nature of only one has been determined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).