ELISA CXC-chemokine receptor 7 (CXCR7)

Reactivity: (Homo sapiens) UniProt:P25106 Abbreviation:CXCR7 Alternative Names:CMKOR1; GPR159; RDC1; G protein-coupled receptor|chemokine orphan receptor 1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.056 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?8.9% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate CXCR7 in samples. An antibody specific for CXCR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCXCR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for CXCR7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCR7 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:C-X-C chemokine receptor type 7 is a member of the G-protein coupled receptor family. Althoµgh this protein was earlier thoµght to be a receptor for vasoactive intestinal peptide (VIP) and was considered to be an orphan receptor, it has been later been shown to bind the chemokines CXCL12/SDF-1 and CXCL11. The protein is also a coreceptor for immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. Alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. Whereas some reports claim that the receptor induces signaling following ligand binding, recent findings in zebrafish sµggest that CXCR7 functions primarily by sequestering the chemokine CXCL12. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).