ELISA Mouse Galectin 3 (GAL-3)

Reactivity:Mouse (Mus muscµLus) UniProt:P16110 Abbreviation:LGALS3 Alternative Names:CBP35; GAL3; GALBP; GALIG; LGALS2; MAC2; IgE-binding protein|MAC-2 antigen|carbohydrate-binding protein 35|galactose-specific lectin 3|galectin 3|galectin-3 internal|laminin-binding protein Application:ELISA Range:15.6-1000 pg/mL Sensitivity:5.9 pg/mL Intra-AssayCV:?5.1% Inter-AssayCV:?9.6% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LGALS3 in samples. An antibody specific for LGALS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LGALS3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Galectin-3 contains a carboxyl-terminal lectin domain and an amino-terminal non-lectin part consisting primarily of short tandem repeats. It is widely distributed in tissues and found in epithelial cells, fibroblasts, dendritic cells, and inflammatory cells. In many cell types studied, galectin-3 is present diffusely in the cytoplasm, but is also localized to the nucleus and subcellµLar structures, such as mitochondria, phagosomes and exosomes, under specific conditions. It is secreted by various cell types, including monocytes, macrophages, and epithelial cells, and the extracellµLar protein can bind to a large number of different glycoconjµgates on the cell surfaces and extracellµLar matrices. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).