ELISA Cation-dependent mannose-6-phosphate receptor (M6PR)

Reactivity: (Homo sapiens) UniProt:P20645 Abbreviation:M6PR Alternative Names:CD-MPR; FLJ32994; MPR46; SMPR; 46-kDa mannose 6-phosphate receptor|Mr 46;000 Man6PR|cation-dependent mannose-6-phosphate receptor Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.63 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.2% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate M6PR in samples. An antibody specific for M6PR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyM6PR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for M6PR is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M6PR bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:M6PR is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellµLar targeting of lysosomal enzymes and export of newly syntheQuantityd lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticµLum, endosomes, and the plasma membrane. Two distinct mannose 6-phosphate receptors have been identified: one is cation-independent and has an apparent molecµLar weight of 215,000. The second, the cation-dependent mannose 6-phosphate receptor, or small mannose 6-phosphate receptor, has an apparent molecµLar weight of only 46,000 and requires divalent cations for high-affinity binding with its ligand. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).