ELISA P2Y purinoceptor 1 (P2RY1)

Reactivity: (Homo sapiens) UniProt:P47900 Abbreviation:P2RY1 Alternative Names:P2Y1; ATP receptor|P2 purinoceptor subtype Y1|P2Y purinoceptor 1|platelet ADP receptor|purinergic receptor P2Y1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.055 ng/mL Intra-AssayCV:?6.2% Inter-AssayCV:?8.5% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate P2RY1 in samples. An antibody specific for P2RY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP2RY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for P2RY1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P2RY1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:P2RY1 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellµLar ATP and ADP. In platelets binding to ADP leads to mobilization of intracellµLar calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. The pharmacologic properties of the P2Y1 receptor are similar to those of the P2T ADP receptor that is responsible for platelet aggregation. They showed that the P2Y1 receptor is expressed by platelets and megakaryoblasts. The authors sµggested that the P2Y1 receptor may be the P2T receptor. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).