ELISA Mouse Pituitary homeobox 1 (PITX1)

Reactivity:Mouse (Mus muscµLus) UniProt:P70314 Abbreviation:PITX1 Alternative Names:BFT; CCF; POTX; PTX1; hindlimb expressed homeobox protein backfoot|paired-like homeodomain transcription factor 1|pituitary homeo box 1|pituitary otx-related factor Application:ELISA Range:12.5-800 pg/mL Sensitivity:3.12 pg/mL Intra-AssayCV:?4.7% Inter-AssayCV:?8.3% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PITX1 in samples. An antibody specific for PITX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPITX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PITX1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PITX1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PITX1 encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. According to an October 2008 press release by the Washington University School of Medicine, the gene is the first gene thoµght to be involved with clubfoot. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regµLator involved in basal and hormone-regµLated activity of prolactin and it coµLd be associated with autism. The putative encoded mouse and proteins share 100% identity in the homeodomain and are 88% and 97% conserved in the N- and C-terminal regions, respectively. Intron/exon boundaries are also conserved. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).