ELISA Zinc finger protein PLAGL1 (PLAGL1)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:Q2I689 Abbreviation:PLAGL1 Alternative Names:DKFZp781P1017; LOT1; MGC126275; MGC126276; ZAC; ZAC1; OTTHUMP00000017344|OTTHUMP00000017345|OTTHUMP00000017346|OTTHUMP00000017347|PLAG-like 1|ZAC tumor supressor|pleiomorphic adenoma gene-like prote Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.064 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?8.1% Recovery:0.86 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PLAGL1 in samples. An antibody specific for PLAGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PLAGL1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PLAGL1 encodes a C2H2 zinc finger protein with transactivation and DNA-binding activity. This gene has been shown to exhibit antiproliferative activities and is a tumor suppressor gene candidate. Many transcript variants encoding two different isoforms have been found for this gene.The deduced 667-amino acid protein contains 7 C2H2 zinc fingers in its N-terminal half. Northern blot analysis of several mouse tissues detected highest Zac1 expression in pituitary.Rat and LOT1 share 67.7% amino acid identity overall, with 85.5% identity in the N-terminal zinc finger region. Northern blot analysis detected mµLtiple LOT1 transcripts in all tissues examined, including spleen, thymus, prostate, testis, ovary, intestine, colon, and leukocytes. Highest expression was detected in ovary. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).