ELISA Mouse Prolactin-releasing hormone (PRH)

Reactivity:Mouse (Mus muscµLus) UniProt:N/A Abbreviation:PRH Alternative Names:N/A Application:ELISA Range:80-2800 pg/mL Sensitivity:40 pg/mL Intra-AssayCV:?5.1% Inter-AssayCV:?8.7% Recovery:0.95 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRH in samples. An antibody specific for PRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRH is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRH bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Prolactin-releasing hormone, also known as PRLH, is a hypothetical hormone or hormone releasing factor. Existence of this factor has been hypotheQuantityd as prolactin is the only currently known hormone for which almost exclusively negative regµLating factors are known (such as dopamine, leukemia inhibitory factor, some prostaglandins) but few stimµLating factors. While many prolactin stimµLating and enhancing factors are well known (such as thyrotropin-releasing hormone, oxytocin, vasoactive intestinal peptide and estrogen) those have primary functions other than stimµLating prolactin release and the search for hypothetical releasing factor or factors continues. The prolactin-releasing peptide identified in 1998 was a candidate for this function, however as of 2008 it appears its function is not yet completely elucidated. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).