ELISA Mouse 43 kDa receptor-associated protein of the synapse (RAPSN)

Reactivity:Mouse (Mus muscµLus) UniProt:P12672 Abbreviation:RAPSN Alternative Names:CMS1D; CMS1E; MGC3597; RNF205; 43 kD receptor-associated protein of the synapse|43 kda postsynaptic protein|acetylcholine receptor-associated 43 kda protein|rapsyn|receptor-associated protein of the Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.55 ng/mL Intra-AssayCV:?6.7% Inter-AssayCV:?10.5% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RAPSN in samples. An antibody specific for RAPSN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAPSN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RAPSN is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPSN bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:43 kDa receptor-associated protein of the synapse is a protein belongs to a family of proteins that are receptor associated proteins of the synapse. It contains a conserved cAMP-dependent protein kinase phosphorylation site. It is believed to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Two splice variants have been identified for this gene. Thoµght to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).