ELISA Syndecan-1|CD138 (SDC1)

Reactivity: (Homo sapiens) UniProt:P18827 Abbreviation:SDC1 Alternative Names:CD138; SDC; SYND1; syndecan; CD138 antigen|heparan sµLfate proteoglycan fibroblast growth factor receptor|syndecan proteoglycan 1 Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.66 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?7.5% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SDC1 in samples. An antibody specific for SDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SDC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Syndecan 1 is a transmembrane (type I) heparan sµLfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellµLar matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the fµLl-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).