ELISA Sheep Sodium-dependent phosphate transport protein 2A (SLC34A1)

Reactivity:Sheep (Ovis aries) UniProt:O97704 Abbreviation:SLC34A1 Alternative Names:NAPI-3; NPHLOP1; NPT2; NPTIIa; SLC11; SLC17A2; Na+-phosphate cotransporter type II|renal sodium-dependent phosphate transporter|sodium/phosphate co-transporter|solute carrier family 17 (sodium phosp Application:ELISA Range:0.31-20 ng/mL Sensitivity:0.1 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?7.5% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC34A1 in samples. An antibody specific for SLC34A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC34A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLC34A1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC34A1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Magagnin et al. (1993) cloned from kidney cortex a cDNA encoding a renal proximal tubµLar, brush-border membrane Na(+)-phosphate cotransporter referred to as NaPi3. Hartmann et al. (1996) reported the cloning and characterization of the mouse (Npt2) and (NPT2) genes. The genes were cloned by screening mouse genomic and chromosome 5-specific libraries, respectively.Hartmann et al. (1996) determined that the mouse and NPT2 genes are approximately 16 kb and contain 13 exons Putative CAAT and TATA boxes were located, respectively, at positions -147 and -40 of the Npt2 gene and -143 and -51 of the NPT2 gene, relative to nucleotide 1 of the corresponding cDNAs. The translation initiation site was within exon 2 of both genes. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).