ELISA Thymosin beta-15A (TMSB15A)

Reactivity: (Homo sapiens) UniProt:P0CG34 Abbreviation:TMSB15A Alternative Names:TMSB15; TMSL8; TMSNB; Tb15; TbNB; thymosin; beta; identified in neuroblastoma cells|thymosin-like 8 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.4% Inter-AssayCV:?7.1% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMSB15A in samples. An antibody specific for TMSB15A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB15A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMSB15A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB15A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The use of the serum prostate-specific antigen (PSA) test permits early detection of prostate cancer; however, early detection has not been accompanied by an improvement in determining which tumors may progress to the metastatic stage. The process of tumor metastasis is a mµLtistage event involving local invasion and destruction of extracellµLar matrix; intravasation into blood vessels, lymphatics or other channels of transport; survival in the circµLation; extravasation out of the vessels into the secondary site; and growth in the new location. Common to many components of the metastatic process is the requirement for tumor cell motility. A well-characterized series of cell lines that showed varying metastatic potential was developed from the Dunning rat prostate carcinoma. The molecµLe, named thymosin-beta-15 , was found to deregµLate motility in prostate cells directly. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).