ELISA Mouse Transient receptor potential cation channel subfamily V member 2 (TRPV2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9WTR1 Abbreviation:TRPV2 Alternative Names:MGC12549; VRL; VRL-1; VRL1; vanilloid receptor-like protein 1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.049 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.2% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TRPV2 in samples. An antibody specific for TRPV2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPV2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TRPV2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPV2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TRPV2 encodes an ion channel that is activated by high temperatures above 52 °C. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thoµght that in other tissues the channel may be activated by stimµLi other than heat.TRPV2 is activated by high temperatures above 52 °C. Alternatively it can be activated at lower temperatures by chemicals, such as the research tool 2-APB, the plant cannabinoid cannabidiol, and probenecid. It is blocked by ruthenium red and lanthanum. TRPV2 was independently discovered by two research groups and described in 1999. It was identified in the lab of David JµLius as a close homolog of TRPV1, the first identified thermosensitive ion channel. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).