ELISA Rat Tubby protein homolog (TUB)

Reactivity:Rat (Rattus norvegicus) UniProt:O88808 Abbreviation:TUB Alternative Names:rd5; tubby|tubby homologue Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.112 ng/mL Intra-AssayCV:?4.8% Inter-AssayCV:?9.6% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TUB in samples. An antibody specific for TUB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TUB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:MicrotubµLes of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubµLins. The genes encoding these microtubµLe constituents belong to the tubµLin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubµLin families are found in all eukaryotes. The alpha and beta tubµLins represent the major components of microtubµLes, while gamma tubµLin plays a critical role in the nucleation of microtubµLe assembly. TUBa1A encodes alpha tubµLin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubµLin genes in a cluster on chromosome 12q. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).