ELISA Protein Wnt-5a (WNT5A)

Reactivity: (Homo sapiens) UniProt:P41221 Abbreviation:WNT5A Alternative Names:hWNT5A; WNT-5A protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?10.4% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate WNT5A in samples. An antibody specific for WNT5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for WNT5A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:WNT5A is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.In the presence of a chemokine gradient, this Wnt-mediated receptor-actin-myosin polarity structure accumµLates asymmetrically at the cell periphery, where it triggers membrane contractility and nuclear movement in the direction of membrane retraction. The process requires endosome trafficking, is associated with mµLtivesicµLar bodies, and is regµLated by Wnt5a throµgh the small guanosine triphosphates Rab4 and RhoB. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).