ELISA Transcriptional repressor protein YY1 (YY1)

Reactivity: (Homo sapiens) UniProt:P25490 Abbreviation:YY1 Alternative Names:DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1; INO80 complex subunit S|Yin and Yang 1 protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.061 ng/mL Intra-AssayCV:?7.1% Inter-AssayCV:?10.4% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate YY1 in samples. An antibody specific for YY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for YY1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YY1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. MµLtifunctional transcription factor that exhibits positive and negative control on a large number of cellµLar and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).