ELISA Phospho-Vimentin (Ser83) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:VIM GeneID:7431 Uniprot:P08670 Specificity:Phospho-Vimentin (Ser83) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser83. Immunogen:A syntheQuantityd peptide derived from Vimentin around the phosphorylation site of Ser83. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction:Pig,Bovine,Sheep,Rabbit,Dog Application:WB,IHC Dilution:WB 1:500-1:2000, IHC 1:50-1:200 Conjµgate:Unconjµgated RRID:AB_2845446 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF2433-BP Des# Blocking Peptide:Phospho-Vimentin (Ser83) Blocking peptide Blocking Peptide Price:$549 CAD