ELISA Rat Acyl-CoA desaturase 1 (Scd1-Scd)

Quantity:96T. Other Quantitys are also available. Please Inquire. Reactivity:Rat (Rattus norvegicus) UniProt:N/A Abbreviation:SCD1 Alternative Names:N/A Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.60 ng/mL Intra-AssayCV:≤6.2% Inter-AssayCV:≤7.6% Recovery:1,01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SCD1 in samples. An antibody specific for SCD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SCD1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCD1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regµLation of cell growth and differentiation throµgh effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 throµgh Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5 (MIM 608370), have been identified in SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).