ELISA Mouse Triggering receptor expressed on myeloid cells 2 (TREM2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q99NH8 Abbreviation:TREM2 Alternative Names:TREM-2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on monocytes 2|triggering receptor expressed on myeloid cells 2a Application:ELISA Range:15.6-1000 pg/mL Sensitivity:6.5 pg/mL Intra-AssayCV:?5.6% Inter-AssayCV:?7.9% Recovery:0.87 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TREM2 in samples. An antibody specific for TREM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTREM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TREM2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREM2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TREmL1 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 and TREM2, but it has distinct structural and functional properties. TREmL1 enhances calcium signaling in an SHP2 (PTPN11)-dependent manner. TLT1 has a calcµLated molecµLar mass of 33 kD. Unlike other TREM proteins, the IgV domain of TLT1 has no potential N-glycosylation sites. The transmembrane domain of TLT1 lacks charged residues, and the 127-amino acid cytoplasmic region has 2 tyr residues (Y245 and Y281), each in an ITIM.truncation resµLts in a membrane-bound form of TLT1 lacking the ITIMs. RT-PCR analysis detected weak expression of TLT1 in monocyte, B-cell, and T-cell lines. TLT1 expression coµLd be upregµLated by mitogen stimµLation. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).