Methylamp DNA Modification Kit

A complete set of optimized reagents designed to convert DNA so that 5-methylcytosines can be detected in downstream applications, for the purpose of gene-specific DNA methylation analysis. The kit has the following advantages and features: • Total protocol time is less than 2 hours. • 99.9% conversion rate of unmethylated cytosine into uracil/thymine. • Extremely low degradation of DNA in the modification process, preventing over 90% of DNA loss. • Requires as little as only 50 pg of input DNA, equivalent to about 20 cells. • Extremely simple, robust, and reliable sodium bisulfite conversion protocol. • Suitable for bisulfite sequencing (direct and cloning), next-generation sequencing, pyrosequencing, PCR (real-time, end-point, methylation-specific), CoBRA (combined bisulfite restriction analysis), and methylation-based microarrays. • Highly compatible with Illumina-based workflows.