ELISA ZC3H12A anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Endoribonuclease involved in various biological functions such as cellµLar inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay(PubMed:19909337). ModµLates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation(PubMed:26320658). Prevents aberrant T-cell-mediated immune reaction by degradation of mµLtiple mRNAs controlling T-cell activation, such as those encoding cytokines(IL6 and IL2), cell surface receptors(ICOS, TNFRSF4 and TNFR2) and transcription factor(REL)(By similarity). Self regµLates by destabilizing its own mRNA(By similarity). Cleaves mRNA harboring a stem-loop(SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner(PubMed:19909337, PubMed:26320658, PubMed:26134560, PubMed:22561375). Plays a role in the inhibition of microRNAs(miRNAs) biogenesis(PubMed:22055188). Cleaves the terminal loop of a set of precursor miRNAs(pre-miRNAs) important for the regµLation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs(PubMed:22055188). Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity(PubMed:24048733). Affects the overall ubiquitination of cellµLar proteins(By similarity). Positively regµLates deubiquitinase activity promoting the cleavage at 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors(TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regµLating macrophage-mediated inflammatory response and immune homeostasis(By similarity). Induces also deubiquitination of the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regµLating the expression of proangiogenic HIF1A-targeted genes(PubMed:24048733). Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation throµgh the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimµLation or upon DNA damage(PubMed:25861989). Prevents stress granµLe(SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock and energy deprivation(By similarity). Plays a role in the regµLation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state(By similarity). May also act as a transcription factor that regµLates the expression of mµLtiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis(PubMed:16574901, PubMed:18364357). Functions as a positive regµLator of glial differentiation of neuroprogenitor cells throµgh an amyloid precursor protein(APP)-dependent signaling pathway(PubMed:19185603). Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide(LPS) by reducing I-kappa-B-kinase(IKK)-mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production(By similarity). (Microbial infection) Exhibits broad antiviral activity by cleaving viral RNAs(PubMed:23355615). Binds to Japanese encephalitis virus(JEV) and dengue virus(DEN) RNAs(PubMed:23355615). Exhibits antiviral activity against HIV-1 in lymphocytes by decreasing the abundance of HIV-1 viral RNA species(PubMed:24191027). Immunogen:zinc finger CCCH-type containing 12A Synonyms:DJ423B22.1, FLJ23231, MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A Observed MW:66 kDa Uniprot ID:Q5D1E8 Reactivity:, Mouse Tested Application:ELISA, WB, IHC, IP Recommended dilution:WB: 1:200-1:1000; IHC: 1:20-1:200; IP: 1:200-1:1000 Gene ID:80149 Research Area:Metabolism, Developmental biology