ELISA Phospho-IRF3 (Ser386) anti-

Quantity :100µL Clone Number: Aliases:IIAE7 antibody; Interferon regµLatory factor 3 antibody; IRF 3 antibody; IRF-3 antibody; IRF3 antibody; IRF3_ antibody; MGC94729 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q14653 Immunogen:Peptide sequence around phosphorylation site of Serine 386(A-S-S(p)-L-E) derived from IRF-3. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB; WB:1:500-1:1000 Background: Key transcriptional regµLator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. RegµLates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimµLated genes (ISG) by binding to an interferon-stimµLated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995). The MGC Project Team; Genome Res. 14:2121-2127(2004). Bellingham J., Ann. Hum. Genet. 62:231-234(1998). Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:IRF3 Research Areas:Epigenetics and Nuclear Signaling;Immunology