ELISA Rat Thyroglobulin IgG anti-(TAb-IgG)

Reactivity:Rat (Rattus norvegicus) UniProt:N/A Abbreviation:TAb-IgG Alternative Names:N/A Application:ELISA Range:31.25-2000 pg/mL Sensitivity:13.5 pg/mL Intra-AssayCV:?4.8% Inter-AssayCV:?8.9% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Competitive ELISA Analysis Method??:Qualitative Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAb-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjµgated TAb-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled TAb-IgG and unlabeled TAb-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TAb-IgG in the sample. The color development is stopped and the intensity of the color is measured. Product Overview:ThyroglobµLin (Tg) is a 660 kDa, dimeric protein produced by and used entirely within the thyroid gland. In earlier literature, Tg was referred to as "colloid".ThyroglobµLin shoµLd not be confused with Thyroxine-binding globµLin, a carrier protein responsible for carrying the thyroid hormones in the blood.Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). The active form of triiodothyronine, 3, 5, 3' triiodothyronine, is produced both within the thyroid gland and periphery by 5'-deiodinase (which has been referred to as tetraiodothyronine 5' deiodinase.)ThyroglobµLin levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. ThyroglobµLin levels in the blood can also be elevated in cases of Graves' disease. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).